Kinetics of Phosphate Incorporation into Adenosine Triphosphate and Guanosine Triphosphate in Bacteria
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چکیده
منابع مشابه
Adenosine Triphosphate : Guanosine Monophosphate Phosphotransferase
Several laboratories have reported studies on individual enzymes that belong to the general class of adenosine triphosphate : nucleoside monophosphate phosphotransferases (EC 2.7.4.4), commonly termed nucleoside monophosphate kinases (l-7). Studies with partially purified enzyme preparations have indicated that a variety of nucleoside monophosphates can serve as alternative substrates for nucle...
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Radioactive adenosine triphosphate was synthesized transiently from adenosine diphosphate and radioactive inorganic phosphate by sodium and potassium adenosine triphosphatase from guinea pig kidney. In a first step, K+-sensitive phosphoenzyme was formed from radioactive inorganic phosphate in the presence of magnesium ion and 16 mM sodium ion. In a second step the addition to the phosphoenzyme ...
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We have previously described an enzyme system from rat liver which catalyzes the incorporation of labeled amino acids into proteins under anaerobic conditions (2, 3). This system consists of two liver cell fractions, the soluble cell fraction and the microsomes, to which are added a labeled amino acid, adenosine triphosphate,l and an ATP-generating system, such as phosphocreatine and creatine k...
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Factors affecting inhibition of myosin ATPase by ATP, such as concentration of the enzyme, of ATP, and of the divalent metals, calcium and magnesium, have been studied and the results are reported here. Experiments were carried out under conditions in which no known magnesium effects occurred, so that only the effect of calcium on the nucleoside triphosphatase activity of myosin A could be qual...
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In recent years, attention has been focussed on the acceptor function of the serine hydroxyl in various enzymes. Acyl or phosphoryl residues appear to link to it in group transfer, and also in the initial phase of ester or peptide hydrolysis (l-4). This has led to a revival of interest in the classical phosphoproteins, vitellic acid and casein, which carry phosphate on the hydroxyl group of ser...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1973
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.116.3.1113-1123.1973